target mouse p815 plasmacytoma cells  (ATCC)

Journal: International journal of cancer

Article Title: Xenogeneic immunization in mice using HER2 DNA delivered by an adenoviral vector.

doi: 10.1002/ijc.20536

Figure Lengend Snippet: FIGURE 3 – CTL response in Ad5-HER2-immunized mice. (a) Spleen cells from BALB/c mice vaccinated with Ad5-HER2 were restimulated in vitro with HER2-15 or HER2-301 peptides and tested for cytotoxic activity against P815 target cells pulsed with the same peptides. As negative control, HER2-15- or HER2-301-restimulated spleen cells were tested on DMSO-pulsed P815 target cells. Average values are reported. (b) Spleen cells from neuT mice vaccinated with Ad5-HER2 were restimulated in vitro with HER2-15.3, neu-15.3, or HER2-301 and tested for cytotoxic activity against P815 target cells pulsed with the same peptides or DMSO (negative control). Spleen cells restimulated by infection with recombinant vaccinia virus ex- pressing r-p185 protein (vv-neu) were tested on target cells pulsed with neu-15.3 peptide or DMSO (negative control). Spleen cells from nonvaccinated mice reproducibly yielded background values of counts. The percentage of specific lysis was calculated by using the following formula: % specific lysis 100 [(experimental release spontaneous release)/(maximum release spontaneous release)], where the spontaneous and maximum release refer to the number of counts in DMSO or in 1% Triton X-100 target cell lysate, respectively. Effector cell/target cell ratios are indicated in the abscissa. The per- centage of specific killing is reported on the vertical axis. Average value of negative controls is displayed. Reported data are representa- tive of several independent experiments.

Article Snippet: Mouse liver NMuLi and target mouse P815 plasmacytoma cells were obtained from the American Type Culture Collection (ATCC, Rockville, MD), Per.C6 cells were from Crucell (Leiden, The Netherlands) and mouse muscle myoblasts C2C7 from G. Abbreviations: A, absorbance; Ad5, adenovirus 5; AP, alkaline phosphatase; CMI, cell-mediated immune; ECD, extracellular domain; ES, electrical stimulation; HER2, human epidermal growth factor receptor 2; ICS, intracellular staining; MCF, mean channel fluorescence; MMTVLTR, mouse mammary tumor virus long terminal repeat; MOI, multiplicity of infection; neu, rat homolog of HER2; p185, protein encoded by HER2; PCNA, proliferating cell nuclear antigen; r-p185, protein encoded by neu; SEB, staphylococcus enterotoxin B; SFC, spot forming cell; vp, viral particle.

Techniques: In Vitro, Activity Assay, Negative Control, Infection, Recombinant, Virus, Lysis

Journal:

Article Title: Fish oil feeding enhances lymphocyte proliferation but impairs virus-specific T lymphocyte cytotoxicity in mice following challenge with influenza virus

doi: 10.1046/j.1365-2249.2000.01135.x

Figure Lengend Snippet: Influenza virus-specific lung cytotoxic T cell activity in mice. The mice were fed diets containing fish oil (□) or beef tallow (▪) (20 g/100 g) for 14 days before intranasal challenge with A/Queensland influenza virus. Cytotoxic T cell activity was determined at 50:1 and 100:1 E:T ratios with influenza virus-infected P815 target cells. Values are mean specific lysis activity ± s.e.m. (n = 5/diet group) at day 5. *Significantly higher cytotoxic activity (P < 0.05).

Article Snippet: Mouse plasmacytoma P815 target cells (American Type Culture Collection (ATCC), Rockville, MD) were labelled with Na 2 51 CrO 4 (3.7 MBq/2 × 10 6 cells; Amersham Int., Aylesbury, UK) for 1 h at 37°C in a 5% CO 2 atmosphere.

Techniques: Activity Assay, Infection, Lysis

Journal: International Journal of Cancer

Article Title: Increased IL-12 P40 homodimer secretion by spleen cells duringin vivo growth of the BW-19 T cell hybridoma accompanies suppression of natural immunity

doi: 10.1002/(sici)1097-0215(19980729)77:3<460::aid-ijc24>3.0.co;2-a

Figure Lengend Snippet: FIGURE 3 – (a) Sensitivity of the hybridoma cells to lysis by poly I:C activated spleen cells, compared with the NK-sensitive lymphosarcoma YAC-1 (% specific lysis 6 SD). (b) Sensitivity of the hybridoma cells to lysis by splenic macrophages (activated or non-activated in vitro), compared with the macrophage-sensitive plasmacytoma P815. (c) Lysis of YAC-1 cells by spleen cells from mice inoculated 7 days before with hybridoma cells, compared with lysis by control spleen cells. (d) Lysis of P815 cells by splenic macrophages (activated or non-activated in vitro) from mice inoculated 7 days before with hybridoma cells, compared with lysis by splenic macrophages from control mice.

Article Snippet: The NKsensitive murine YAC-1 lymphoma line and the macrophagesensitive P815 plasmacytoma cell line were purchased from the ATCC (Rockville, MD; ATCC TIB 160 and TIB 64).

Techniques: Lysis, In Vitro, Control